Journal: The International Journal of Neuropsychopharmacology

Article Title: Activation of signaling pathways downstream of the brain-derived neurotrophic factor receptor, TrkB, in the rat brain by vagal nerve stimulation and antidepressant drugs

doi: 10.1017/s1461145713000977

Figure Lengend Snippet: Fig. 6. Western blot analysis of hippocampus CREB (≈50 kDa) phosphorylation at S133 after chronic 14 d VNS, sertraline (7.5 mg/ kg/d i.p.) or DMI (10 mg/kg/d i.p.). *p<0.01, Student’s t-test. The number of rats in each group is shown in the bars.

Article Snippet: Membranes were incubated at 4 °C overnight with the following primary antibodies: anti-pY515 (1:1000 in 2.5% BSA in TBST, Abcam, USA), anti-pY705 TrkB (1:1000 in 2.5% BSA in TBST, Abcam, USA), anti-pY816 TrkB (1:4000 in 2.5% BSA in TBST, Abcam, USA), and anti-TrkB (full length, 1:10000 in 2.5% BSA in TBST, Neuromics, USA), anti-PLCγ1 (1:1000), anti-PLC γ1 Y783 (1:250 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pERK T202/Y204 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-ERK (1:2000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-AKT (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pAKT T308 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-CREB (1:2000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti-pCREB S133 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA), anti- pp70 S6 kinase T389 (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA) and anti-p70 S6 kinase (1:1000 in 5% BSA in TBST, Cell Signaling Technology, Inc, USA).

Techniques: Western Blot, Phospho-proteomics